Genetically Encoded, pH-Sensitive mTFP1 Biosensor for Probing Lysosomal pH
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چکیده
منابع مشابه
Illumination of the Spatial Order of Intracellular pH by Genetically Encoded pH-Sensitive Sensors
Fluorescent proteins have been extensively used for engineering genetically encoded sensors that can monitor levels of ions, enzyme activities, redox potential, and metabolites. Certain fluorescent proteins possess specific pH-dependent spectroscopic features, and thus can be used as indicators of intracellular pH. Moreover, concatenated pH-sensitive proteins with target proteins pin the pH sen...
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Changes in pH are now widely accepted as a signalling mechanism in cells. In plants, proton pumps in the plasma membrane and tonoplast play a key role in regulation of intracellular pH homeostasis and maintenance of transmembrane proton gradients. Proton transport in response to external stimuli can be expected to be finely regulated spatially and temporally. With the ambition to follow such ch...
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Fluorescent proteins (FPs) have given access to a large choice of live imaging techniques and have thereby profoundly modified our view of plant cells. Together with technological improvement of imaging, they have opened the possibility to monitor physico-chemical changes within cells. For this purpose, a new generation of FPs has been engineered. For instance, pHluorin, a point mutated version...
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ژورنال
عنوان ژورنال: ACS Sensors
سال: 2021
ISSN: 2379-3694,2379-3694
DOI: 10.1021/acssensors.0c02318